It is feasible that rDNA gene activity in PHA-stimulated lymphocytes is augmented in patients with adenocarcinoma due to the presence of plasma mediators arising from cellular malignancies.
Seven tissue-specific isozymes--lactate dehydrogenases A and B (LDHA and LDHB), esterases B2 and A4 (ESB2 and ESA4), creatine kinase B (CKB), and red blood cell and tissue forms of adenosine deaminases (ADA)--were examined in the human adenocarcinoma cell line LS174T and its subclones grown under a wide variety of conditions.
Seven tissue-specific isozymes--lactate dehydrogenases A and B (LDHA and LDHB), esterases B2 and A4 (ESB2 and ESA4), creatine kinase B (CKB), and red blood cell and tissue forms of adenosine deaminases (ADA)--were examined in the human adenocarcinoma cell line LS174T and its subclones grown under a wide variety of conditions.
Seven tissue-specific isozymes--lactate dehydrogenases A and B (LDHA and LDHB), esterases B2 and A4 (ESB2 and ESA4), creatine kinase B (CKB), and red blood cell and tissue forms of adenosine deaminases (ADA)--were examined in the human adenocarcinoma cell line LS174T and its subclones grown under a wide variety of conditions.
Standard carcinoembryonic antigen(s) (CEA) obtained commercially, and CEA preparations from cell membranes and spent media of cultured human malignant melanotic melanoma (HMMC-ShAc and HMMC-ShAm) and of human colon adenocarcinomas (Levo c, Levo m, SW-403 c and SW-403 m) suppressed human peripheral blood lymphocyte (PBL).
We have found that the expression of p53 messenger RNA is growth regulated in human cells following kinetics similar to that previously shown in mouse 3T3 cells, and is increased in the large majority of colon adenocarcinomas in comparison to adjacent normal mucosa and adenoma.
Thus, the protein products of the amplified c-erbB-2 gene may have a role in the evolution of adenocarcinomas, as does the EGF receptor in some squamous-cell carcinomas.
Two (PC-8 and PC-9) of the adenocarcinomas overproduced EGF receptor mRNA and protein, and exhibited gene amplification, the magnitude of which was comparable to that of A431 cells.
Among patients with pulmonary adenocarcinoma the frequency of the Hp 2-2 type was significantly (p less than 0.05) lower compared to the controls, with a corresponding increase of the Hp 1-1 and Hp 2-1 types.
The use of immunohistological staining to detect the level of expression of the c-erbB-2 protein in human breast adenocarcinomas may be of value in predicting tumour behaviour.
Southern blot analysis of a subset of the tumours detected amplification of the EGF receptor gene in squamous cell carcinomas but not in adenocarcinomas.
A survey of 225 human tumors for changes involving AKT1 led to the discovery of a 20-fold amplification of this gene in one of the five gastric adenocarcinomas tested.
We have found that normal human kidney, six of eight adenocarcinomas of the kidney, and four cell lines derived from kidney adenocarcinomas express high levels of mdr1 mRNA.
We have found that normal human kidney, six of eight adenocarcinomas of the kidney, and four cell lines derived from kidney adenocarcinomas express high levels of mdr1 mRNA.