mgrA mutants displayed significantly higher susceptibility to HDPs, which might be related to the decreased HDP-induced mprF and dltA expression but decreased Fn and EC adherence, EC damage, α-toxin production, agr RNAIII, hla and fnbA expression, and attenuated virulence in the bacteremia model as compared to their respective parental and mgrA-complemented strains.
The fibronectin-binding protein A gene (<i>fnbpA</i>) was sequenced.Of 2,365 cases of SAB, 92 had IE.After matching, 37 pairs of <i>S. aureus</i> isolates from the IE cases and non-IE controls were compared; <i>fnbpA</i> was detected in 91.9% of the IE isolates and 100% of the non-IE isolates (<i>p</i> = 0.24).
The persistent bacteremia isolates (n = 5) formed significantly stronger bonds with immobilized fibronectin as determined by dynamic binding measurements performed with atomic force microscopy.
We analysed bacterial isolates from 34 patients with S. aureus bacteraemia and implanted cardiac devices for association with CDI, FnBPA sequence, classification into a clonal complex (CC), and binding to fibronectin (Fn).