Emerging evidence suggests a pivotal role of lipin-1 as a crucial downstream regulator of SIRT1-AMPK signaling system that is likely to be ultimately responsible for development and progression of AFLD.
C57BL/6 mice were fed an ethanol diet (ED) to induce AFL, or a control diet (CD) for 6weeks, and then subjected to 60min of ischemia and 5h of reperfusion.2-ME2 (15mg/kg, i.p.) was administered 12h before ischemia and 10min before reperfusion, and sirtinol (sirtuin 1 (SIRT1) inhibitor, 10mg/kg, i.p.) was administered 30min before reperfusion.