After SPINT2 knockdown and knock-in in adult and pediatric HGG cell lines, a variety of in vitro assays was carried out to determine the role of SPINT2 in glioma cell viability and invasion, as well as their mechanistic associations with metalloprotease activities.
We identified SPINT2 as a candidate tumor-suppressor gene within a group of CpG islands (designated G<sub>T</sub>-CMG) that are hypermethylated in both IDH1<sup>MUT</sup> and IDH1<sup>WT</sup> gliomas but not in normal brain.
Because the expression of HAI-2/B is inversely related to glioma invasiveness and degree of malignancy, this finding may provide insight into glioma initiation and progression as well as potentially providing new therapeutic targets.
To further explore the possible role of HAI-2/PB in glioma progression, cultured human glioblastoma cell lines (U251 and YKG-1) were transiently transfected with an expression vector harboring human HAI-2/PB cDNA.