This bone loss was associated with an acute decrease in transcript abundance for <i>Acp5, Nos2, Arg1</i> and this decrease persisted for <i>Nos2</i> and <i>Arg1</i>.
Compared with the OVX group, ZGW groups showed significantly reduced levels of serum tartrate-resistant acid phosphatase 5b (TRACP-5b) and β-cross-linked c-telopeptide of type I collagen (β-CTX) (P ＜ 0.01), increased levels of serum bone-specific alkaline phosphatase (BALP) (P ＜ 0.01) and OPG (P ＜ 0.05), prevention of OVX-induced bone loss, and improved microarchitecture of the trabecular bone of distal femur.
Here we demonstrate that matrine significantly prevented ovariectomy-induced bone loss and inhibited osteoclastogenesis <i>in vivo</i> with decreased serum levels of TRAcp5b, TNF-α, and IL-6.
Notably, MCP-1<sup>-/-</sup> mice were protected against PTH-induced cortical and trabecular bone loss as well as from increases in serum CTX (C-terminal crosslinking telopeptide of type I collagen) and TRACP-5b (tartrate-resistant acid phosphatase 5b).