Our results showed that symptoms of anxiety, depression and sleep disturbances were not associated with S. invicta stings (for GAD-7 scale, Pearson Chi-Square test, χ2 = 0.152, df = 1, P = 0.697; for the BAI, χ2 = 2.252, df = 1, P = 0.133; for the PHQ-9, χ2 = 0.098, df = 1, P = 0.754; for the PSQI(5), χ2 = 0.536, df = 1, P = 0.453).