Furthermore, directed by surface electrostatics distribution of t3CrGES and Erg20<sup>WW</sup> (Erg20<sup>F96W-N127W</sup>), co-expression of the reverse fusion of Erg20<sup>ww</sup>/t3CrGES and another copy of Erg20<sup>WW</sup> promoted the geraniol titer to 523.96mg/L at shakes flask level, due to enhancing GPP accessibility led by protein interaction of t3CrGES-Erg20<sup>WW</sup> and the free Erg20<sup>WW</sup>.