To comprehensively prioritise potential MHC-II-binding epitopes from <i>Mycobacterium bovis</i>, the agent of bTB and zoonotic TB in humans, we integrated three binding prediction methods with the <i>M. bovis</i>proteome using a subset of human HLA alleles to approximate the binding of epitope-containing peptides to the bovine MHC class II molecule BoLA-DRB3.