The receiver operating characteristic (ROC) curve showed that the sensitivity, specificity and area under the curve (AUC) of miR-29a in PBMC was 67.20%, 88.50% and 0.852, respectively, and that in CSF was 81.10%, 90.00% and 0.890, respectively, and that in combination of PBMC and CSF was 84.40%, 95.38% and 0.934, respectively. miR-29a expression in PBMC and CSF may provide a reference for the diagnosis of pediatric TBM.