As a result, we observed higher level of XIST and Smurf1, but lower level of miR-27a in SCI rats and lipopolysaccharide (LPS)-induced primary microglial cells. in vitro, LPS induced SCI microglia cells as described by decreased cell viability and B cell lymphoma 2 (Bcl-2) expression, and increased cell apoptosis rate, Bax and cleaved caspase 3 levels, and tumor necrosis factor α (TNF-α) and interleukin 6 (IL-6) secretions. in vivo, a T10 laminectomy caused SCI rats as evidenced by decreased Basso-Beattie-Bresnahan Locomotor Rating Scale (BBB) score and induced expression of Bax, cleaved caspase 3, TNF-α and IL-6.
It was also observed that increased miR‑27a expression promoted osteogenic differentiation and cell proliferation, inhibited caspase‑3/9 and B‑cell lymphoma‑2‑associated X protein expression and induced alkaline phosphatase (ALP) activity and bone morphogenetic protein (BMP)‑2, runt‑related transcription factor (Runx)2 and osteonectin mRNA expression in osteoblastic MC3T3‑E1 cells. miR‑27a mimics also induced transforming growth factor (TGF)‑β and Smad7 protein expression in MC3T3‑E1 cells.
A luciferase assay was performed to evaluate the effect of miR‑27a on phosphatase and tensin homolog (PTEN) and B cell lymphoma (Bcl)‑2 associated X, apoptosis regulator (BAX) expression.