Immunodetection of the proteins VEGF, caspase-3, and p53 is not influenced by smoking or alcohol consumption, suggesting that other molecular mechanisms are associated with the biological aggressiveness of oral carcinoma in patients exposed to these risk factors.
Using specific antibodies a study of the expression and subcellular distribution of Bcl-2, BAX, caspase-3 and cytochrome c in normal human keratinocytes and mouth carcinoma slowly (HN) and rapidly growing (KB) cells has been carried out.
The caspase 3 null parental cell line was further examined in comparison with a well-characterized, caspase 3 wild type oral carcinoma cell line by MTT assay and BrdU incorporation assay.