In this study, using Western blotting and RT-qPCR techniques and the fluorescent splicing reporters, we examined the detailed expression patterns of l- and s-afadin isoforms across various tissues and cell types, including rat organs at developmental stages, primary cultured neuronal and non-neuronal cells prepared from the developing rat brain, and in neurons in vitro generated from P19embryonal carcinoma (EC) cells.
Retinoic acid signaling has been thoroughly interrogated in ECCs, especially in the F9 and P19 murine cell models, and while we have touched on this aspect, this review purposely highlights how some key transcription factors regulate pluripotency and cell stemness prior to this signaling.
Here, we demonstrate that BOC and ABL are induced in P19embryonal carcinoma (EC) cells and cortical neural progenitor cells (NPCs) during neuronal differentiation.
The P19EC cells differentiate to form neurons but the ability of the neurites to extend and make contacts with neighbouring neurites is compromised when treated with the hANG-ALS variants.
In the present study, we used antisera that specifically recognized the active forms of caspase-8, -3, and -9 but not their proforms, and showed that only caspase-8 and -3 were activated during the generation of polyQ72 aggregates in P19EC cell nuclei.
Forced expression of mouse p130 induced growth suppression of P19EC cells and Western analysis of transfected P19 cells suggested the cloned cDNA encodes the full-length p130 protein.
Addition of cell extract from undifferentiated P19embryonal carcinoma (EC) cells preferentially stimulated translation of an IGF-II 5' UTR RNA construct.
These studies demonstrate that P19EC cell differentiation can be divided into LIF sensitive and insensitive pathways which correlate with differentiation of endodermal/mesodermal and neuro-ectodermal cell types, respectively.