We therefore investigated the utility of the gene expression patterns of 12 candidate genes (<i>TLR1</i>, -<i>2</i>, -<i>4</i>, -<i>6</i>, and <i>10</i>, <i>DEFA1</i>, <i>LTF</i>, <i>IL1B</i>, <i>BPI</i>, <i>CRP</i>, <i>IFNG</i>, and <i>DEFB4A</i>) previously associated with infection for detection of PJI in periprosthetic tissues of patients with total joint arthroplasty (TJA) (<i>n</i> = 76) reoperated for PJI (<i>n</i> = 38) or aseptic failure (<i>n</i> = 38), using the ultrafast quantitative reverse transcription-PCR (RT-PCR) Xxpress system (BJS Biotechnologies Ltd.).