Imageological and histological observations showed that the fracture lines of mice in the PBS injection group and the agomiR-NC injection group were found to be healed at 28 d after fractures, while fuzzy fracture lines could be seen in mice with fewer calluses in the agomiR-214-3p injection group, and the expression level of osteocalcin at each time point in the agomiR-214-3p injection group was decreased compared with that in the control group.
On day 24 post-surgery, Ca-SGP remarkably increased the mRNA expression of osteogenesis markers Col1a and OCN, and increased callus BV/TV and Tb.N, this facilitating the formation of woven bone.
In tibiae, bone volume, callus and cortical volume, and gene expression of osteocalcin and alkaline phosphatase were enhanced by zileuton (10 or 100 mg); biomechanical properties and bone density were not changed.