<b>Results</b>: HLA-A*02:01/CHM1<sup>319</sup>-specific allorestricted CD8<sup>+</sup> T cells showed specific <i>in vitro</i> lysis of all patient-derived ES cell lines.
Expanded T cells specifically recognised peptide-pulsed target cells or antigen-transfected cells in the context of HLA-A 0201 and killed HLA-A 0201(+) ET lines expressing the antigen while HLA-A 0201(-) ET lines were not affected.
Two representative ET cell lines with either detectable HLA-A, -B antigens (A673) or absolutely non-detectable HLA-A, -B antigens (SK-ES-1) were further subjected to transcriptional analysis.