The PPI network revealed eight hub genes: Ubiquitin‑60S ribosomal protein L40, Ras‑related C3 botulinum toxin substrate, mitogen‑activated protein kinase 1, epidermal growth factor receptor, cystic fibrosis transmembrane conductance regulator, PI3K regulatory subunit 1, AKT1, and actin‑related protein 2/3 complex subunit 1A, which may be the key target genes of miR‑542‑3p in OS.
In addition, transfection of CD99wt inhibits the expression of several molecules crucial to the remodelling of the actin cytoskeleton, such as ACTR2, ARPC1A, Rho-associated, coiled-coil containing protein kinase 2 (ROCK2) as well as ezrin, an ezrin/radixin/moesin family member that has been clearly associated with tumour progression and metastatic spread in osteosarcoma.