The aim of the present study was to analyze early and late activation of T-lymphocytes related to Epstein-Barr virus (EBV) infection by the expression of markers of activation (CD69, CD25) on the surface of T-lymphocytes (CD3<sup>+</sup>, CD4<sup>+</sup>, CD8<sup>+</sup>) in patients bearing laryngeal cancer according to absence/presence immunoglobulin G antibodies to EBV nuclear antigen (EBNA1).
Then, AMC-HN-8 cells were transfected with miR-141 mimic, miR-141 inhibitor and HOXC6-siRNA to investigate specific role of miR-141, HOXC6 and TGF-β signaling pathway in laryngeal cancer in vivo and in vitro.
Then, AMC-HN-8 cells were transfected with miR-141 mimic, miR-141 inhibitor and HOXC6-siRNA to investigate specific role of miR-141, HOXC6 and TGF-β signaling pathway in laryngeal cancer in vivo and in vitro.
The aim of the present study was to analyze early and late activation of T-lymphocytes related to Epstein-Barr virus (EBV) infection by the expression of markers of activation (CD69, CD25) on the surface of T-lymphocytes (CD3<sup>+</sup>, CD4<sup>+</sup>, CD8<sup>+</sup>) in patients bearing laryngeal cancer according to absence/presence immunoglobulin G antibodies to EBV nuclear antigen (EBNA1).
To identify the putative relevance of autophagy in laryngeal cancer, we performed an immunohistochemistry study to analyze the expression of the proteins involved in this process, namely, LC3, ATG5 and p62/SQSTM1.
The blood serum levels of MMP-2, MMP-9, MMP-7, and TIMP-2 in patients with laryngeal cancer and 100 healthy subjects were measured using the enzyme-linked immunosorbent assay (ELISA) method.
Overall, our results suggest that i) cytoplasmic p62 and PTOV1 can be considered prognostic markers in laryngeal cancer, ii) the acquisition of resistance seems to be related to PTOV1 and autophagy-related protein overexpression, iii) by increasing autophagy, PTOV1 might contribute to resistance in this model and iv) the expression of autophagy-related proteins could classify a subgroup of laryngeal cancer patients who will benefit from a therapy based upon autophagy inhibition.
Accordingly, western blot assay suggested that the expression of DKK1, vimentin and β-catenin was significantly decreased after YAP downregulated treatment, thereby indicating that YAP mediated the EMT programme and the Wnt/β-catenin signalling pathway in carcinoma of the larynx.
The risk coefficients in the multivariate Cox analysis revealed that LINC02154 and MNX1-AS1 are risk factors for laryngeal cancer, whereas MYHAS and LINC01281 appear to be protective factors.
Overall, our results suggest that i) cytoplasmic p62 and PTOV1 can be considered prognostic markers in laryngeal cancer, ii) the acquisition of resistance seems to be related to PTOV1 and autophagy-related protein overexpression, iii) by increasing autophagy, PTOV1 might contribute to resistance in this model and iv) the expression of autophagy-related proteins could classify a subgroup of laryngeal cancer patients who will benefit from a therapy based upon autophagy inhibition.
Furthermore, restored TCF-4 expression rescued the inhibitory roles of miR-379 overexpression on cell proliferation, migration, and invasion.Our study for the first time demonstrated that miR-379/TCF-4 might involve in the progression of laryngeal carcinoma, and miR-379 appears to serve as a novel tumor suppressor in laryngeal carcinoma.
Small Nucleolar RNA Host Gene 12 (SNHG12) Promotes Proliferation and Invasion of Laryngeal Cancer Cells via Sponging miR-129-5p and Potentiating WW Domain-Containing E3 Ubiquitin Protein Ligase 1 (WWP1) Expression.
<b>Result:</b> The effective adhesion rate of MB<sub>MMP-1</sub> and MB<sub>IgG</sub> to HEp-2 was 298.42 ± 16.57 versus 12.38 ± 3.26 bubbles/per field <i>in vitro</i> experiment, which shows a significant difference (<i>P</i> < 0.01).
Overall, our results suggest that i) cytoplasmic p62 and PTOV1 can be considered prognostic markers in laryngeal cancer, ii) the acquisition of resistance seems to be related to PTOV1 and autophagy-related protein overexpression, iii) by increasing autophagy, PTOV1 might contribute to resistance in this model and iv) the expression of autophagy-related proteins could classify a subgroup of laryngeal cancer patients who will benefit from a therapy based upon autophagy inhibition.
Overall, our results suggest that i) cytoplasmic p62 and PTOV1 can be considered prognostic markers in laryngeal cancer, ii) the acquisition of resistance seems to be related to PTOV1 and autophagy-related protein overexpression, iii) by increasing autophagy, PTOV1 might contribute to resistance in this model and iv) the expression of autophagy-related proteins could classify a subgroup of laryngeal cancer patients who will benefit from a therapy based upon autophagy inhibition.