Noninvasive diagnosis of TRIT1-related mitochondrial disorder by measuring i<sup>6</sup> A37 and ms<sup>2</sup> i<sup>6</sup> A37 modifications in tRNAs from blood and urine samples.
Matchmaking facilitates the diagnosis of an autosomal-recessive mitochondrial disease caused by biallelic mutation of the tRNA isopentenyltransferase (TRIT1) gene.