4, STS 2B, HDPE, and PP coupons were treated with SS at 100°C and SHS at 125 and 150°C for 2, 4, 7, 10, 15, and 20 s. Among all coupon types, SHS was more effective than SS in inactivating the <i>S. aureus</i> biofilms.<i>S. aureus</i> biofilms on steel coupons were more susceptible to most SS and SHS treatments than the biofilms on plastic coupons.<i>S. aureus</i> biofilms on HDPE and PP coupons were reduced by 4.00 and 5.22 log CFU per coupon, respectively, after SS treatment (100°C) for 20 s. SS treatment for 20 s reduced the amount of <i>S. aureus</i> biofilm on STS No.