We aimed to determine if the interaction of MTHFD1synthetase deficiency and low folate intake increases developmental abnormalities in a mouse model for MTHFD1 R653Q.
We induced tumor formation in a mouse model for MTHFD1-synthetase deficiency (Mthfd1S<sup>+/-</sup> ) using combined administration of azoxymethane (AOM) and dextran sodium sulfate (DSS) in male and female wild-type and Mthfd1S<sup>+/-</sup> mice.