With over-expression and knockdown of miR-190b in WT-derived cell line SK-NEP-1, we next studied cell proliferation, cell circle, apoptosis, invasion and migration abilities change caused by miR-190b ectopic expression.
We describe the use of gene expression profiling to show that SK-NEP-1, a cell line previously thought to represent anaplastic Wilms tumor, is instead related to Ewing sarcoma.
Moreover, HACE1 expression was virtually undetectable in the SK-NEP-1Wilms' tumor cell line and in four of five additional primary Wilms' tumor cases compared with patient-matched normal kidney.