To determine if IPMK was upstream of integrin β1 expression, we examined IPMK<sup>-/-</sup> mouse embryonic fibroblast cells and found that integrins β1 and β3 gene expression was reduced by half, relative to wild-type cells, whereas focal adhesion kinase (FAK) activity and Rho/Rac/Cdc42 protein levels were increased, resulting in migration defects.