This method will provide investigators with a relatively simple tool to non-invasively examine the role of altered hepatic mitochondrial metabolism.Liver mitochondrial metabolism plays an important role for glucose and lipid homeostasis and its alterations contribute to metabolic disorders, including fatty liver and diabetes.Here Perry et al. develop a method for the measurement of hepatic fluxes by using lactate and glucose tracers in combination with NMR spectroscopy.
Metabolic profiling of ob/ob mouse fatty liver using HR-MAS <sup>1</sup>H-NMR combined with gene expression analysis reveals alterations in betaine metabolism and the transsulfuration pathway.