In vitro cytoxicity assay and apoptosis analysis showed that oMCNs-RES induced enhanced cytotoxic effect and pro-apoptosis effect mediated via the PARP and Caspase-3 protein cleavage in TNBC cell line, respectively.
<i>In silico</i> analysis suggested that <i>OTUD7B</i> regulation, probably owing to miR-1180 downregulation, may negatively regulate the NF-κB-Lin28 axis which in turn triggers Let-7 microRNA-mediated caspase-3 downregulation, thereby conferring paclitaxel resistance in TNBCs.
We also found a significant decrease of caspase-3 and p53 expression after 48 h, accompanied by a down-regulation of NF-κB in cells exposed to MWCNT-COOH-CDDP system which promotes apoptosis escape and thus failing to overcome the triple negative breast cancer (TNBC) cells resistance.
Vaccination increased type I T cells in the tumor (<i>P</i> = 0.001) and decreased cells expressing the stem cell marker, Sca-1, compared with controls (<i>P</i> = 0.004).<b>Conclusions:</b> An HIF-1α vaccine may be uniquely effective in limiting tumor growth in TNBC.
In the present study, we observed that DSF/Cu treatment induced apoptosis, mediated by caspase-3 activation in triple-negative breast cancer (TNBC) cells.