Genetic variants close to genes that encode crucial enzymes for the synthesis (<i>DHCR7</i> rs12785878), metabolism (<i>CYP2R1</i> rs2060793) and degradation (<i>CYP24A1</i> rs6013897) of vitamin D have been associated with serum levels of vitamin D. The aim of this case-control study was to determine the effect of these variants in the vitamin D pathway on the susceptibility to thyroid cancer.