In this context, a genetic association study was carried out between two eNOS polymorphisms (the ecNOS4a/b VNTR and the G894T substitution) in a sample of 101 nuclear families having one affected offspring of ischemic heart disease (IHD).
Using robust family-based association tests specifically designed for the study of complex diseases, we found no evidence that the G894T polymorphism of the eNOS gene has a significant role in the development of IHD in our population.
The GluAsp or AspAsp genotype of the Glu298Asp polymorphism was significantly associated with IHD, but not albuminuria in these Japanese diabetic subjects.