rs121912438
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0.100 |
GeneticVariation |
BEFREE |
We observed an increased total iron content in G93A-SOD1 SH-SY5Y neuroblastoma cells compared to wild-type (WT)-SOD1 cells. mRNA expression for transferrin receptor 1 (TfR1) and divalent metal transporter 1 was increased in G93A-SOD1 cells, which was in accordance with higher iron uptake.
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23178912 |
2013 |
rs121912438
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0.100 |
GeneticVariation |
BEFREE |
Overexpression of a familial mutant form of superoxide dismutase 1 (SOD1) (G93A) in neuroblastoma cells resulted in a similar reduction of IGF-1Rβ protein.
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22875931 |
2012 |
rs121912438
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0.100 |
GeneticVariation |
BEFREE |
To characterize the cellular response to mitochondrial perturbations at the level of gene expression and alternative pre-mRNA splicing we used splicing-sensitive microarrays to profile human neuroblastoma SH-SY5Y cells treated with paraquat, a neurotoxic herbicide that induces the formation of reactive oxygen species and causes mitochondrial damage in animal models, and SH-SY5Y cells stably expressing the mutant G93A-SOD1 protein, one of the genetic causes of ALS.
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21120952 |
2011 |
rs121912438
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0.100 |
GeneticVariation |
BEFREE |
Impairment of nuclear factor-kappaB activation increased glutamate excitotoxicity in a motoneuron-neuroblastoma hybrid cell line expressing mutant (G93A) Cu/Zn-superoxide dismutase.
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20623531 |
2010 |
rs121912438
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|
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0.100 |
GeneticVariation |
BEFREE |
To understand better the role of these mutations in the pathophysiology of FALS we have compared the pattern of proteins expressed in human neuroblastoma SH-SY5Y cell line with those of cell lines transfected with plasmids expressing the wild-type human SOD1 and the H46R and G93A mutants.
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17979159 |
2007 |
rs121912438
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|
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0.100 |
GeneticVariation |
BEFREE |
Treatment of G93A-SOD</span>1 SH-SY5Y neuroblastoma cells with the antioxidant N-acetylcysteine reduced the toxicity of pneumolysin.
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17997855 |
2007 |
rs121912438
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0.100 |
GeneticVariation |
BEFREE |
We detected this interaction both in spinal cord extracts of mutant SOD1(G93A) transgenic mice and in cultured neuroblastoma cells.
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17403032 |
2007 |
rs121912438
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0.100 |
GeneticVariation |
BEFREE |
In order to investigate the basis of the tissue specificity of mutant SOD1 we compared the effect of the continuous expression of wild-type or mutant (G93A) human SOD1 on mitochondrial morphology in the NSC-34 motoneuronal-like, the N18TG2 neuroblastoma and the non-neuronal Madin-Darby Canine Kidney (MDCK) cell lines.
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16903849 |
2006 |
rs121912438
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0.100 |
GeneticVariation |
BEFREE |
Human neuroblastoma SH-SY5Y cells transfected with either familial amyotrophic lateral sclerosis-typical G93A mutant or wild-type copper/zinc superoxide dismutase were compared to untransfected cells in term of glutamate transport.
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15670639 |
2005 |
rs121912438
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0.100 |
GeneticVariation |
BEFREE |
These effects are consequent to activation of inflammatory processes in G93A-glioblastoma cells stimulated by cocultured G93A-neuroblastoma.
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15208263 |
2004 |
rs121912438
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0.100 |
GeneticVariation |
BEFREE |
We report that the expression of mutant G93A copper/zinc superoxide dismutase (SOD1), associated with familial amyotrophic lateral sclerosis, specifically causes a decrease in MTT reduction rate and ATP levels and an increase in both cytosolic and mitochondrial reactive oxygen species (ROS) production in human neuroblastoma SH-SY5Y cells compared to cells overexpressing wild-type SOD1 and untransfected cells.
|
12901835 |
2003 |
rs121912438
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0.100 |
GeneticVariation |
BEFREE |
In order to investigate the effect of various calcium modulators and the SOD-1 mutation on neuronal death, we tested motoneuron-neuroblastoma hybrid (VSC 4.1) cells constitutively expressing human SOD-1 gene with mutations (A4V, G93A) or wild-type.
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12151755 |
2002 |
rs121912438
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0.100 |
GeneticVariation |
BEFREE |
We have recently observed that calcineurin activity is depressed in human neuroblastoma cells expressing Cu,Zn superoxide dismutase (SOD1) mutant G93A and in brain areas from G93A transgenic mice, and that mutant G93A-SOD1 oxidatively inactivates calcineurin in vitro.
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12437573 |
2002 |
rs121912438
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|
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0.100 |
GeneticVariation |
BEFREE |
We have set up a model system for familial amyotrophic lateral sclerosis (FALS) by transfecting human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase (Cu,ZnSOD) or a mutant of this enzyme (G93A) associated with FALS.
|
9315720 |
1997 |
rs121912431
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0.040 |
GeneticVariation |
BEFREE |
Here, we show that stable overexpression of mutant human SOD1 (G37R) - but not wild-type SOD1 (wt-SOD1) - in mouse neuroblastoma cells (N2a) results in morphological abnormalities of mitochondria accompanied by several dysfunctions.
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21388680 |
2011 |
rs121912431
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0.040 |
GeneticVariation |
BEFREE |
Previously, we reported that overexpression of the mitochondrial antioxidant manganese superoxide dismutase (MnSOD or SOD2) attenuates cytotoxicity induced by expression of the G37R-SOD1 mutant in a human neuroblastoma cell culture model of ALS.
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17394531 |
2007 |
rs121912431
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0.040 |
GeneticVariation |
BEFREE |
Mouse neuroblastoma Neuro-2a cells were transfected with human wild-type or mutated (G37R, G93A) Cu,Zn-SOD.
|
15456693 |
2005 |
rs121912431
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0.040 |
GeneticVariation |
BEFREE |
Expression of mutant SOD1 (G37R) resulted in a time and dose-related death of differentiated neuroblastoma cells.
|
12067230 |
2002 |
rs121912443
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0.030 |
GeneticVariation |
BEFREE |
In the light of the possibility that different SOD1 entities could be expressed also in other neurodegenerative disorders, as a sort of unifying event with AD and PD, we have investigated amyotrophic lateral sclerosis (ALS) using human neuroblastoma SH-SY5Y cells with mutated SOD1 gene H46R as cellular model.
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17987632 |
2007 |
rs121912443
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|
|
0.030 |
GeneticVariation |
BEFREE |
To understand better the role of these mutations in the pathophysiology of FALS we have compared the pattern of proteins expressed in human neuroblastoma SH-SY5Y cell line with those of cell lines transfected with plasmids expressing the wild-type human SOD1 and the H46R and G93A mutants.
|
17979159 |
2007 |
rs121912443
|
|
|
0.030 |
GeneticVariation |
BEFREE |
We have investigated the response to oxidative stress in a model system obtained by stable transfection of the human neuroblastoma cell line SH-SY5Y with plasmids directing constitutive expression of either wild-type human Cu,Zn superoxide dismutase or a mutant of this enzyme (H46R) associated with familial amyotrophic lateral sclerosis.
|
10461909 |
1999 |
rs121912452
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0.010 |
GeneticVariation |
BEFREE |
We also detected oligomer formation of L84V SOD1 in L84V SOD1-expressing human neuroblastoma cells.
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20816908 |
2010 |