The combination of [U-<sup>13</sup>C<sub>3</sub>]glycerol and NMR analysis enabled investigation of multiple biochemical processes in hepatomas and surrounding liver.
In this work, NMR metabolomics has been applied to reveal the metabolic effects of AgNPs toward human hepatoma (HepG2) cells, which are relevant with respect to nanoparticle accumulation and detoxification.
The objective of this study was to investigate the pathway of aspartate metabolism in HCC of humans.Coupled with transcriptomic (i.e. mRNA) and NMR based metabolomics of human tissue extracts, we utilized liquid chromatography mass spectrometry based metabolomics analysis of stable [U-(13) C6 ]glucose metabolism or [U-(13) C5 ,(15) N2 ]glutamine metabolism of HCC cell culture.