|
Carcinoma of lung
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
We have examined 88 lung cancer lines for p16INK4 protein expression and have observed a striking inverse correlation between the presence of p16INK4 and wildtype RB.
|
7936665 |
1994 |
|
Carcinoma of lung
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
CDKN2 gene silencing in lung cancer by DNA hypermethylation and kinetics of p16INK4 protein induction by 5-aza 2'deoxycytidine.
|
7566983 |
1995 |
|
Carcinoma of lung
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
We also examined alterations of these three genes and expression of p16INK4 in 21 human lung cancer cell lines.
|
7882351 |
1995 |
|
Carcinoma of lung
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
One half of all cancer cell lines and one fourth of lung cancer cell lines examined to date contain homozygous deletions (i.e., both alleles lost) of CDKN2.
|
7563154 |
1995 |
|
Carcinoma of lung
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
In summary, inactivation of CDKN2 is implicated in the development of about 20% of NSCLC, but the possibility of another tumor suppressor gene on chromosome segment 9p21 important in lung cancer cannot be eliminated.
|
8589032 |
1995 |
|
Carcinoma of lung
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
To examine its tumor suppressive function and its potential in cancer gene replacement therapy, wild-type p16INK4 was expressed in an adenovirus-derived gene delivery system and introduced into lung cancer cell lines that do not express p16INK4.
|
7614457 |
1995 |
|
Carcinoma of lung
|
0.100 |
Biomarker
|
disease |
BEFREE |
Both copies of the candidate tumor suppressor gene on chromosome 9, CDKN2, are deleted in approximately one-fourth of lung cancer cell lines examined and the protein product of CDKN2, p16 is undetectable in one-third of the lung cancer cell lines studied.
|
7551956 |
1995 |
|
Carcinoma of lung
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Western blot analysis showed that the expression of both NM23 and MTS1 proteins was reduced in human lung cancer CH27 cells by retinoic acid treatment, but the ratio of NM23: MTS1 increased in a dose-dependent manner.
|
9066685 |
1997 |
|
Carcinoma of lung
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
Furthermore, rat lung cancer appears to be an excellent model in which to investigate the mechanisms of de novo gene methylation and the role of p16 dysfunction in the progression of neoplasia.
|
9032263 |
1997 |
|
Carcinoma of lung
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
There is an inverse relationship in lung cancer cells between expression of RB and p16INK4A, an upstream regulator of RB.
|
9508209 |
1998 |
|
Carcinoma of lung
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Association of CDKN2A(p16)/CDKN2B(p15) alterations and homozygous chromosome arm 9p deletions in human lung carcinoma.
|
9624535 |
1998 |
|
Carcinoma of lung
|
0.100 |
Biomarker
|
disease |
BEFREE |
Aberrant methylation of p16(INK4a) is an early event in lung cancer and a potential biomarker for early diagnosis.
|
9751761 |
1998 |
|
Carcinoma of lung
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
A methylenetetrahydrofolate reductase polymorphism is associated with expression of p16 in human lung cancer.
|
9625844 |
1998 |
|
Carcinoma of lung
|
0.100 |
Biomarker
|
disease |
BEFREE |
These results are consistent with an alternative growth suppressor function for hp19ARF in NE lung cancer that is distinct from that of p16INK4a.
|
9731504 |
1998 |
|
Carcinoma of lung
|
0.100 |
Biomarker
|
disease |
BEFREE |
These observations suggest the value of adenovirally-mediated p16INK4a gene replacement therapy for lung cancer.
|
9858892 |
1998 |
|
Carcinoma of lung
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
In contrast, cancer cell lines which were wild-type for p16 but mutant or null for pRb (Saos-2, C33a and H358) were <threefold more sensitive to Adp16 when compared to a control virus.
|
9464545 |
1998 |
|
Carcinoma of lung
|
0.100 |
Biomarker
|
disease |
BEFREE |
Since somatic mutations of the p53, RB and p16 genes occur frequently in lung cancer and the replication error phenotype is seen in a subset of lung cancer, it is possible that germ-line mutations of the p53, RB, p16 and mismatch repair genes influence the susceptibility to lung cancer.
|
9614442 |
1998 |
|
Carcinoma of lung
|
0.100 |
Biomarker
|
disease |
BEFREE |
Coincidental alterations of p16INK4A/CDKN2 and other genes in human lung cancer cell lines.
|
9673367 |
1998 |
|
Carcinoma of lung
|
0.100 |
Biomarker
|
disease |
BEFREE |
These results indicate that strain-specific variation in p16INK4a function is exploited in mouse lung tumorigenesis and strongly implicate a role for p16INK4a in lung cancer predisposition and development.
|
10365910 |
1999 |
|
Carcinoma of lung
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
The known cellular functions of the tumor suppressor genes most commonly affected in lung cancer are reviewed herein, including the retinoblastoma (Rb) gene on chromosome 13q14, the p53 gene on 17p13, and the cyclin-dependent kinase inhibitor (CDKN2) gene on 9p21.
|
9891228 |
1999 |
|
Carcinoma of lung
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Polymerase chain reaction amplification and direct sequencing of p16 exons 1 and 2 revealed no mutations, indicating that p16-altered expression in lung cancer is not necessarily linked to mutational events of these genes.
|
10469971 |
1999 |
|
Carcinoma of lung
|
0.100 |
Biomarker
|
disease |
BEFREE |
High concentrations of recombinant adenovirus expressing p16 gene induces apoptosis in lung cancer cell lines.
|
9891478 |
1999 |
|
Carcinoma of lung
|
0.100 |
Biomarker
|
disease |
BEFREE |
In contrast, p16 expression was lost in moderate dysplasia (12%) and in CIS (30%) in patients with lung cancer. p16 loss occurred exclusively in patients who displayed loss of p16 expression in their related invasive carcinoma.
|
10037171 |
1999 |
|
Carcinoma of lung
|
0.100 |
Biomarker
|
disease |
BEFREE |
We transfected p16INK4A gene into lung cancer cell lines which do not express p16 protein.
|
10063314 |
1999 |
|
Carcinoma of lung
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
Hypermethylation of the p16INK4 gene was reported to occur at an early stage in lung cancer.
|
11106248 |
2000 |