In the control group all the parosteal osteosarcomas (five of five, 100%) were FRS2 amplified, whereas the remaining 103 samples were FRS2 non-amplified.
In total, 761 overlapping genes in 987 CNV regions and in the genes in 7,313 miRNA-gene pairs were obtained. miRNAs (hsa-miR-27a-3p, hsa-miR-124-3p, hsa-miR-9-5p, hsa-miR-182-5p, hsa-miR-26a-5p) and the genes [Fibroblast growth factor receptor substrate 2 (<i>FRS2</i>), coronin 1C (<i>CORO1C</i>), forkhead box P1 (<i>FOXP1</i>), cytoplasmic polyadenylation element binding protein 4 (<i>CPEB4</i>) and glucocorticoid induced 1 (<i>GLCCI1</i>)] with the highest degrees of association with osteosarcoma development were identified.