Compared with normal controls, expression levels of <i>collagen type VI alpha 3 chain</i> (<i>COL6A3)</i>, <i>serpin family H member 1 (SERPINH1)</i> and <i>pleckstrin homology and RhoGEF domain containing G1 (PLEKHG1)</i> were significantly upregulated in GC plasmas.
RT-PCR analysis using divergent primers, RNase R digestion assay, and mRNA stability assay were performed to characterize circCOL6A3 (ID: hsa_circ_0006401) in GC cell lines; Western blot was conducted to detect the expression of COL6A3.
When a representative predictor set of 92 genes was examined, pathways of 'focal adhesion' (with gene components of THBS2, PDGFD, MAPK1, COL1A2, COL6A3), 'ECM-receptor interaction' pathway (THBS2, COL1A2, COL6A3, FN1) and 'TGF-beta signaling' (THBS2, MAPK1, INHBA) represent some of the main differences between normal and tumor of gastric cancer at a molecular level.