Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Here, we knocked down TDP-43 in murine neuroblastoma Neuro2A cells and found a time lag between efficient TDP-43 depletion and appearance of cell death, suggesting that several mechanisms mediate between these two events.
|
29125873 |
2017 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Lastly, we demonstrated that HSC70 silencing directly increases TDP-43 protein levels in human neuroblastoma cells.
|
31663379 |
2020 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
In the human neuroblastoma SK-N-MC cell line, exogenous PROG (9300-93,000 pg/ml) had a significant effect on overall Tau and nuclear TDP-43 levels, reducing total Tau levels by ∼1.5-fold and increasing nuclear TDP-43 by 1.7- to 2.0-fold.
|
23798570 |
2013 |
Central neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Similar suppression of TDP-43 provoked the downregulation of GAD67, the Gad1 homolog protein in human neuroblastoma cell lines and analogous modifications were observed in iPSC-derived motoneurons from patients carrying mutations in TDP-43, uncovering conserved pathological mechanisms behind the disease.
|
29379112 |
2018 |
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Ribosomal loss of RACK1, which excludes TDP-43 from the translational machinery, remarkably reduces formation of TDP-43 cytoplasmic inclusions in neuroblastoma cells.
|
28158562 |
2017 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Importantly, we found TDP43 expression was required for TRIM16-induced inhibition of neuroblastoma and breast cancer cell growth and the repressive effect of TRIM16 on cell cycle regulatory proteins, E2F1 and pRb.
|
26902425 |
2016 |
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Here we show that the internalization of human pre-formed TDP-43 aggregates in the murine neuroblastoma N2a cells promptly resulted in their ubiquitination and hyperphosphorylation by endogenous machineries, mimicking the post-translational modifications observed in patients.
|
31357627 |
2019 |
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
SOD1 misfolding and oxidation was also detected using immunocytochemistry and quantitative immunoprecipitation of human neuroblastoma SH-SY5Y cells as well as cultured murine spinal neural cells transgenic for human wtSOD1, which were transiently transfected with human cytosolic mutant FUS or TDP43, or wtTDP43.
|
22493728 |
2012 |
Central neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
To this end, we have firstly identified RNA targets of TDP-43 using UV-Cross-Linking and Immunoprecipitation (UV-CLIP) of SHSY5Y cells, a human neuroblastoma cell line.
|
21421050 |
2011 |
Central neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
We also focus on in vitro models comprised of cultured SH-SY5Y neuroblastoma cells expressing mutant forms of transactive response DNA binding protein 43 kDa (TDP-43) and showing a reduced number of TDP-43 inclusion-containing cells upon Dimebon treatment along with activation of autophagy markers.
|
26123670 |
2015 |
Central neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
In the current study, we analyzed protein turnover and subcellular distribution of wild-type TDP-43 and two disease-associated mutants (G298S and A382T) in human neuroblastoma SH-SY5Y cells stably expressing TDP-43 with a C-terminal tag.
|
24477737 |
2014 |