|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
However, the hepatoma line PLC/PRF/5, which contains 2,300 IL6 receptors, was more sensitive to IL6-PE40 (amount of protein required to inhibit protein synthesis by 50% was 5 ng/ml) than both the myeloma cell lines U266 and H929 (for both cell lines, the 50% inhibitory dose was 8 ng/ml), which contain 15,500 and 16,500 IL6 receptors, respectively.
|
2160579 |
1990 |
|
Liver neoplasms
|
0.100 |
GeneticVariation
|
group |
BEFREE |
Twenty-seven IL-6 variants were tested for biological activity on a human hepatoma and a mouse hybridoma cell line.
|
1321818 |
1992 |
|
Liver neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
However, we observed that SOCS1-deficient mice developed numerous and large liver tumor nodules following treatment with the hepatocarcinogen diethylnitrosamine (DEN) without showing increased interleukin-6 production or activation of p53.
|
26725321 |
2016 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
The response of HepG2 cells to IL-6 stimulation was significantly affected by heat treatment at 40 degreesC.
|
9755236 |
1998 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
The structure-function relationships of the biological activities of mutant varieties of the pleiotropic cytokine interleukin-6 (human) were measured by three assays: induction of immunoglobulin M (IgM) secretion from an Epstein-Barr virus-transformed human B cell line and induction of fibrinogen secretion from either a human hepatoma cell line or a rat hepatoma cell line.
|
1328443 |
1992 |
|
Liver neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
HBx was overexpressed in hepatic and hepatoma cell lines and IL-6 expression levels were measured by quantitative RT-PCR and ELISA.
|
20937539 |
2011 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
The aims of this study are to address if insulin is anti-inflammatory and attenuates IL-6-induced inflammation in the human hepatoma cell line HepG2 and if this involves signal transducer and activator of transcription 3 (STAT3) signal transduction.
|
21298325 |
2011 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
(b) Among peptides covering the entire sequence of human IL-6 and the corresponding antipeptide antibodies, the peptide IL-6[35-66] and anti-IL-6[35-66] most effectively inhibited the interaction between human hepatoma HepG2 cells and the preS(21-47) ligand, suggesting that this region of the human IL-6 sequence encompasses a binding site for the HBV env protein.
|
1334115 |
1992 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Here we have treated the human hepatoma cell line Hep 3B with either crude MoCM or recombinant IL-6 and compared the effect of the two stimulants on the expression of both endogenous AP genes and recombinant plasmids introduced into the cells by transfection.
|
2457587 |
1988 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Stable transfection of IL-6-cDNA into HepG2 cells (HepG2-IL-6) resulting in constitutive secretion of 2 micrograms of IL-6 per 10(6) cells in 24 h led to a down-regulation of surface-bound gp80 and subsequent homologous desensitization of HepG2-IL-6 cells towards IL-6.
|
1321738 |
1992 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Interleukin-6 production by human hepatoma lines is related to a low degree of cell differentiation.
|
8210716 |
1993 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
This study on a human hepatoma cell line and primary rat hepatocytes examined how the effectiveness of IL-6 is modified by the presence of soluble IL-6 receptor and whether the sIL-6R in the absence of IL-6 acts on hepatocytes.
|
9722935 |
1998 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
The induced expression of GBP28 was also observed in human hepatoma HepG2 cells after treatment with IL-6.
|
11444852 |
2001 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Activin B also induces Smad1/5/8 phosphorylation in human hepatoma-derived cells and, synergistically with IL-6 and STAT-3 signaling, up-regulates hepcidin expression markedly, an observation confirmed in mouse primary hepatocytes.
|
22611157 |
2012 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
We have analyzed the regulation of PAI-1 mRNA accumulation by interleukin (IL)-1, IL-6, and dexamethasone, known mediators of the acute phase response, in HepG2 cells, a highly differentiated human hepatoma cell line that produces a broad spectrum of acute phase proteins including PAI-1.
|
8034668 |
1994 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
We report here that interleukin-6 (IL-6), the principal cytokine mediating the synthesis of most acute-phase proteins in the liver, downregulates the production of factor XII by the human hepatoma cell line HepG2 by up to 75%.
|
9269767 |
1997 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Affinity cross-linking of 125I-labeled recombinant human interleukin-6 (IL-6) to human hepatoma cells (HepG2) allowed the detection of three IL-6-containing complexes with molecular masses of 100 kDa, 120 kDa and 200 kDa.
|
2163835 |
1990 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
The transcription rate of the haptoglobin (Hp) gene is stimulated by interleukin-1 (IL-1), IL-6, and dexamethasone in rat hepatoma (H-35) cells.
|
2172789 |
1990 |
|
Liver neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
The protein levels of interleukin-6 (IL-6) and vascular endothelial growth factor A (VEGF-A) were persistently elevated in orthotopic liver tumors, but not in subcutaneous tumors, treated with sorafenib.
|
29266245 |
2018 |
|
Liver neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
In this study using reverse transcriptase polymerase chain reaction (RT-PCR) we isolated and characterised a truncated form of amplified cDNA reverse-transcribed from IL-6 receptor mRNA both from human hepatoma cell line HepG2 and mononuclear cells from inflammatory bowel disease (IBD) patients.
|
10397166 |
1999 |
|
Liver neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
Moreover, we found significantly decreased expression of IL-6 in the spleen as well as decreased NF-κB in the paraventricular nucleus of rats with Yoshida ascites hepatoma.
|
31606594 |
2019 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Peptides were tested alone or in combination with recombinant hIL-6 on an IL-6 responsive human hepatoma HepG2 cell line.
|
11405230 |
2001 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
A biological consequence of enhanced secretion of IFN-beta 2/IL-6 was the ability of media from infected FS-4 cell cultures to enhance by 8-15-fold the synthesis and secretion of a typical acute phase plasma protein (alpha 1-antichymotrypsin) by human hepatoma Hep3B2 cells.
|
3133443 |
1988 |
|
Liver neoplasms
|
0.100 |
Biomarker
|
group |
BEFREE |
Among these, IL-6DBP/NF-IL6 plays a key role in IL-6 signal transduction because its trans-activation potential is induced by IL-6 in the human hepatoma cell line Hep3B.
|
7680115 |
1993 |
|
Liver neoplasms
|
0.100 |
AlteredExpression
|
group |
BEFREE |
To study the mechanism of interleukin-6 (IL-6) induction of human C-reactive protein (CRP) gene expression, we have utilized a human hepatoma (PLC/PRF/5) cell culture system to analyze the trans-acting factors which bind to the 300 bp 5'-flanking region of human CRP gene.
|
8703909 |
1996 |