In conclusion, the results of the present study suggest that the SNHG20/miR-140-5p/NDRG3 axis may be involved in mediating resistance to 5-FU in gastric cancer.
SGC-7901 cells were treated with miR-140-5p mimic/inhibitor, siRNA against <i>THY1</i> and siRNA against <i>Notch1</i> in order to determine their regulatory roles in GC cell activities.
The aim of the current study was to investigate the role and molecular mechanisms of miR‑140 in Helicobacter pylori (Hp)‑associated gastric cancer, and to examine its relationship with immune function in gastric cancer.
Gain- and loss-of-function assays revealed that increased miR-140-5p expression significantly inhibited GC cell proliferation and invasion ability, as well as the Wnt/β-catenin signaling pathway by decreasing WNT1 and β-catenin expression.
Real-time PCR, western-blot, MTT assay, and flow cytometry cell cycle analysis were utilized to explore the molecular pathway of miR-140 involved in the progression of GC.
The configured biomarker panel consisted of 3 mRNAs (<i>SPINK7</i>, <i>PPL</i>, and <i>SEMA4B</i>) and 2 miRNAs (<i>MIR140-5p</i> and <i>MIR301a</i>), which were all significantly down-regulated in the GC group, and yielded an area under the ROC curve (AUC) of 0.81 (95% CI, 0.72-0.89).
miR-140-5p serves as a potential prognostic factor in patients with GC, and miR-140-5p mediated YES1 inhibition is a novel mechanism behind the suppressive effects of miR-140-5p in GC.