|
Glioblastoma
|
0.500 |
GeneticVariation
|
disease |
UNIPROT |
|
|
|
|
Glioblastoma
|
0.500 |
GeneticVariation
|
disease |
BEFREE |
In preliminary screens, mutations of PTEN were detected in 31% (13/42) of glioblastoma cell lines and xenografts, 100% (4/4) of prostate cancer cell lines, 6% (4/65) of breast cancer cell lines and xenografts, and 17% (3/18) of primary glioblastomas.
|
9072974 |
1997 |
|
Glioblastoma
|
0.500 |
AlteredExpression
|
disease |
BEFREE |
Thus, PTEN appears to be the major target of inactivation on chromosome 10q in glioblastoma multiforme.
|
9331071 |
1997 |
|
Glioblastoma
|
0.500 |
GeneticVariation
|
disease |
BEFREE |
Of 124 tumor specimens exhibiting LOH that have been screened for MMAC1 alterations to date, we have detected variants in 13 (approximately 10%) of these primary tumors; the highest frequency of variants was found in glioblastoma specimens (approximately 23%).
|
9393738 |
1997 |
|
Glioblastoma
|
0.500 |
GeneticVariation
|
disease |
BEFREE |
Competitive PCR assays were developed to address the possible occurrence of PTEN/MMAC1 homozygous deletions in glioblastomas, and this analysis identified three samples having loss of both PTEN/MMAC1 alleles.
|
9393744 |
1997 |
|
Glioblastoma
|
0.500 |
Biomarker
|
disease |
BEFREE |
Most recently, a putative tumor suppressor gene (PTEN/MMAC1) has been identified in the region between D10S215 and an adjacent, more telomeric marker (D10S541) and was found to be altered in breast cancers, prostate cancers, and glioblastomas.
|
9491329 |
1998 |
|
Glioblastoma
|
0.500 |
AlteredExpression
|
disease |
BEFREE |
Here, we show that PTEN expression potently suppressed the growth and tumorigenicity of human glioblastoma U87MG cells.
|
9860981 |
1998 |
|
Glioblastoma
|
0.500 |
Biomarker
|
disease |
BEFREE |
Molecular analysis of two putative tumour suppressor genes, PTEN and DMBT, which have been implicated in glioblastoma multiforme disease progression.
|
9796706 |
1998 |
|
Glioblastoma
|
0.500 |
Biomarker
|
disease |
BEFREE |
These findings confirm that PTEN is one of the chromosome 10 tumor suppressor genes involved in the development of glioblastomas.
|
9619835 |
1998 |
|
Glioblastoma
|
0.500 |
GeneticVariation
|
disease |
BEFREE |
PTEN mutations were detected in 9 of 36 glioblastomas (25%).
|
9426052 |
1998 |
|
Glioblastoma
|
0.500 |
Biomarker
|
disease |
BEFREE |
These data indicate that PTEN/MMAC1 is inactivated in a subset of GBM and suggest that the high mutation frequency previously found in GBM established cell lines reflects culture condition artifacts rather than the true mutation frequency in vivo.
|
9484844 |
1998 |
|
Glioblastoma
|
0.500 |
GeneticVariation
|
disease |
BEFREE |
Infection of MMAC1-mutated U87MG glioblastoma cells with MMCB resulted in dose-dependent exogenous MMAC1 protein expression as detected by Western blotting of cell lysates.
|
9622068 |
1998 |
|
Glioblastoma
|
0.500 |
GeneticVariation
|
disease |
BEFREE |
The observation that secondary glioblastomas have a p53 mutation as a genetic hallmark but rarely contain a PTEN mutation supports the concept that primary and secondary glioblastomas develop differently on a genetic level.
|
9690672 |
1998 |
|
Glioblastoma
|
0.500 |
GeneticVariation
|
disease |
BEFREE |
The final progression process leading to glioblastoma multiforme seems to need additional genetic abnormalities in the long arm of chromosome 10; one of which is deletion and/or functional loss of the PTEN/MMAC1 gene.
|
11550308 |
1999 |
|
Glioblastoma
|
0.500 |
GeneticVariation
|
disease |
BEFREE |
Further somatic PTEN mutations have been found in glioblastomas and to a lesser extent in meningiomas.
|
10371336 |
1999 |
|
Glioblastoma
|
0.500 |
Biomarker
|
disease |
BEFREE |
It has been shown in glioblastoma cell lines that loss of chromosome 10q, where the PTEN gene is located, is associated with increased angiogenic activity in the conditioned medium attributable to downregulation of thrombospondin-1, a negative regulator of angiogenesis.
|
10208463 |
1999 |
|
Glioblastoma
|
0.500 |
Biomarker
|
disease |
BEFREE |
PTEN is a candidate tumor suppressor gene identified on human chromosome 10q23.3 that is frequently mutated or deleted in 30% to 44% of glioblastomas.
|
10331435 |
1999 |
|
Glioblastoma
|
0.500 |
Biomarker
|
disease |
BEFREE |
Expression of carboxyl-terminal mutants in PTEN-deficient glioblastoma cells permitted the anchorage-independent growth of the cells that otherwise was suppressed by wild-type PTEN.
|
10468583 |
1999 |
|
Glioblastoma
|
0.500 |
AlteredExpression
|
disease |
BEFREE |
Therefore, overexpression of MMAC1 via adenovirus-mediated gene transfer suppresses tumor cell growth through cell cycle inhibitory mechanisms, and as such, represents a potential therapeutic approach to treating glioblastomas.
|
10344736 |
1999 |
|
Glioblastoma
|
0.500 |
GeneticVariation
|
disease |
BEFREE |
We recently reported that PTEN (MMAC1) on 10q23.3 is mutated in approximately 30% of primary (de novo) glioblastomas but rarely in secondary glioblastomas that progressed from low-grade or anaplastic astrocytomas.
|
10433932 |
1999 |
|
Glioblastoma
|
0.500 |
Biomarker
|
disease |
BEFREE |
Thus, loss of wild type PTEN represents one of the major abnormalities associated with astrocytic tumor progression to glioblastoma and provides a strong selective growth advantage when cultivating glioblastoma tissue in xenografts.
|
10560660 |
1999 |
|
Glioblastoma
|
0.500 |
GeneticVariation
|
disease |
BEFREE |
Moreover, they suggest that PTEN alterations are equally involved in the 2 glioblastoma pathways defined by the presence of EGFR amplification and p53 mutation.
|
10096247 |
1999 |
|
Glioblastoma
|
0.500 |
Biomarker
|
disease |
BEFREE |
We found evidence for a PTEN-related sequence (PTEN-rs) on genomic DNA of GBM and non-neoplastic cells.
|
10378766 |
1999 |
|
Glioblastoma
|
0.500 |
Biomarker
|
disease |
BEFREE |
PTEN/MMAC1 (phosphatase and tensin homolog/mutated in multiple advanced cancers 1) is a tumor suppressor gene, the inactivation of which is an important step in the progression of gliomas to end-stage glioblastoma multiforme.
|
11303623 |
2000 |
|
Glioblastoma
|
0.500 |
GeneticVariation
|
disease |
BEFREE |
The psiPTEN expression was complementary to PTEN mutation, for 14 of 18 glioblastomas showed either PTEN mutation or psiPTEN expression and only one case showed both PTEN mutation and psiPTEN expression (P<0.046), suggesting a pathological role of psiPTEN expression as an alternative to PTEN mutation in glioblastomas.
|
10980610 |
2000 |