Adipocyte defects in GDM consistent with abnormalities in GLUT 4-vesicle traffic have implications with respect to potential mechanisms of insulin resistance in human muscle.
Stepwise regression analysis revealed that RBP4 mRNA expression in SAT was independently predicted by GLUT4 mRNA expression (β= 0.59, p = 0.003) and the presence of GDM (β=0.46, p = 0.01), whereas RBP4 mRNA expression in VAT was related to PPARγ mRNA expression (β= 0.64, p = 0.0003) and the patient's age (β= -0.38, p = 0.03).
In the placental tissue of non-obese women, there was a decrease in expression of SHBG protein and mRNA, with a concurrent decrease in expression of GLUT-4 protein and mRNA in women with GDM compared with normal controls.
Morphometric analysis revealed a significant increase in the expression of GLUT-4 and GLUT-9 in insulin-dependent diabetic women (GDMG2 + PGDM) as compared to both, control and GDMG1 groups (p < .05).
The protein density of ARHGEF11 and ROCK1 was positively correlated with birth weight (p < .001) in the NGT groups. p-Y612, PI3K, pAKT and GLUT4 were significantly decreased in NGT-M, GDM-N and GDM-M group (p < .05).