Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Here, we discover aberrant HSF1 degradation by aggregated α-synuclein (or α-synuclein-induced proteotoxic stress) in transfected neuroblastoma cells.
|
26503960 |
2016 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We optimized an automated and reproducible assay to quantify both α-SYN aggregation and cell death in human SH-SY5Y neuroblastoma cells.
|
26620202 |
2016 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Overexpression of αS in male flies as well as in retinoic acid pre-treated neuroblastoma cells led to an elevation of histone H3K9 methylations, mostly mono- (H3K9me1) and di- (H3K9me2).
|
27808254 |
2016 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
In this study, we demonstrate that α-synuclein exists as defined, subcellular-specific species that change characteristics in response to oxidative stress in neuroblastoma cells and in response to Parkinson's disease pathogenesis in human cerebellum and frontal cortex.
|
27837450 |
2017 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Here we show that recombinant expression of alpha-synuclein in human neuroblastoma cells enhances cellular levels of PLCβ1 but blunts its signaling pathway, preventing the agonist-dependent rise of cytoplasmic Ca<sup>2+</sup>.
|
27871937 |
2017 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We also show that squalamine almost completely suppresses the toxicity of α-synuclein oligomers in human neuroblastoma cells by inhibiting their interactions with lipid membranes.
|
28096355 |
2017 |
Childhood Neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Finally, we show that this association inhibits α-synuclein A53T oligomer toxicity in neuroblastoma cells.
|
28102321 |
2017 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Calcipotriol inhibits α-synuclein aggregation in SH-SY5Y neuroblastoma cells by a Calbindin-D28k-dependent mechanism.
|
28164279 |
2017 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Hence, in this study we analysed the influence of these two proteins (α-Syn and wild-type or mutant Atx3) on modified redox signaling, a pathological process potentially linked to both diseases, and also the impact of exposure to iron and rotenone in SH-SY5Y neuroblastoma cells.
|
28236214 |
2017 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We used neuroblastoma SH-SY5Y cell line and rat synaptoneurosomes treated with exogenous soluble ASN.
|
28516276 |
2017 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
WT alpha-synuclein was stably overexpressed in human BE(2)-M17 neuroblastoma cells resulting in increased levels of an alpha-synuclein multimer, but no increase in alpha-synuclein monomer levels.
|
28620826 |
2017 |
Childhood Neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
TH1-S31E associated with vesicular monoamine transporter 2 (VMAT2) and α-synuclein in neuroblastoma cells, and endogenous THSer(P)-31 was detected in VMAT2- and α-synuclein-immunoprecipitated mouse brain samples.
|
28637871 |
2017 |
Childhood Neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Marked dysregulations of microbial defense factors Ifit3 and Rsad2 were consistently observed upon five analyses: (1) Pink1 <sup>-/-</sup> primary neurons in the first weeks after brain dissociation, (2) aged Pink1 <sup>-/-</sup> midbrain with transgenic A53T-alpha-synuclein overexpression, (3) human neuroblastoma cells with PINK1-knockdown and murine Pink1 <sup>-/-</sup> embryonal fibroblasts undergoing acute starvation, (4) triggering mitophagy in these cells with trifluoromethoxy carbonylcyanide phenylhydrazone (FCCP), and (5) subjecting them to pathogenic RNA-analogue poly(I:C).
|
28768533 |
2017 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
To explore CpG methylation associating with α-synuclein transcription and its underlying mechanisms in the neurotoxin-induced PD pathology, human neuroblastoma SH-SY5Y cells were treated with neurotoxins 6-hydroxydopamine (6-OHDA) and 1-methyl-4-phenylpyridinium (MPP<sup>+</sup>).
|
28807729 |
2017 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We show, using a pH-sensitive probe, that internalization of α-synuclein amyloid fibrils in neuroblastoma cells is dependent on heparan sulfate, whereas internalization of smaller non-amyloid oligomers is not.
|
28827536 |
2017 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Generated antibodies recognize misfolded hα-Syn produced by neuroblastoma cells, hα-Syn in the brain tissues of transgenic mouse strains and in the brain tissues of dementia with Lewy body cases.
|
28870518 |
2017 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Moreover, treatment of the hit compounds significantly reduced the α-synuclein expression in SH-SY5Y human neuroblastoma cells, that is implicated in Parkinson's disease.
|
28883518 |
2017 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
In support of these conclusions, mutations that target the region that promotes strong membrane interactions by α-synuclein oligomers suppressed their toxicity in neuroblastoma cells and primary cortical neurons.
|
29242346 |
2017 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Aggregated α-Syn was observed following differentiation of N1E-115 neuroblastoma cells transfected with h-α-Syn.
|
29375308 |
2017 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
We then used a human neuroblastoma cells (M17) stably over-expressing alpha-synuclein and found that RAC1 function decreased the amount of amyloidogenic alpha-synuclein.
|
29429047 |
2018 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Herein we have investigated the perturbations induced by low molecular weight α-synuclein and different types of α-synuclein oligomers in the neuroblastoma SH-SY5Y cells.
|
29539261 |
2018 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Thus, we concluded that α-synuclein inhibited the starvation-induced autophagy in neuroblastoma SH-SY5Y cells via inhibiting the mTOR/Atg 5 signaling.
|
29551576 |
2018 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Likewise, in human neuroblastoma SH-SY5Y cells exposed to low-dose MPP<sup>+</sup> , Nramp1 expression and cathepsin D activity were decreased, along with an increase in α-synuclein protein expression and aggregation.
|
29679389 |
2019 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Propanoylated lysine, a specific indicator of docosahexaenoic acid oxidation, was increased in neuronal differentiated human neuroblastoma SH-SY5Y cells overexpressing α-synuclein.
|
29892158 |
2018 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Real-time determination of aggregated alpha-synuclein induced membrane disruption at neuroblastoma cells using scanning ion conductance microscopy.
|
29974096 |
2018 |