Degenerative polyarthritis
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Tumour necrosis factor alpha (TNF alpha) was produced spontaneously at high levels by both RA and OA synovial cells.
|
2505790 |
1989 |
Degenerative polyarthritis
|
0.100 |
Biomarker
|
disease |
BEFREE |
Synovial fluid (SF) mononuclear cells (MNC) from 13 patients with rheumatoid arthritis (RA) and 12 patients with other arthritic diseases (OD) including osteoarthritis (OA), gout and spondyloarthritis (SA) were cultured in the presence of collagen types I and II or lipopolysaccharide (LPS) for 24 h. Interleukin-1 (IL-1), IL-6 and tumor necrosis factor-alpha (TNF-alpha) in the SF and culture supernatants were assayed using ELISA.
|
7622181 |
1995 |
Degenerative polyarthritis
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
To compare the cytokine profile with the degree and composition of cellular infiltration in rheumatoid arthritis (RA) and osteoarthritis (OA) synovium, synovial membranes from patients with RA (n = 14) and OA (n = 5) were examined, employing immunohistochemistry and competitive reverse-transcriptase polymerase chain reaction (RT-PCR), for interleukin (IL)-I beta, IL-2, IL-4, IL-5, IL-6, and IL-10, and tumour necrosis factor-alpha (TNF-alpha) gene expression.
|
9032818 |
1997 |
Degenerative polyarthritis
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
MMP-7 gene expression in cultured OA chondrocytes was enhanced by the treatment with interleukin-1alpha and/or tumor necrosis factor-alpha.
|
9461124 |
1998 |
Degenerative polyarthritis
|
0.100 |
Biomarker
|
disease |
BEFREE |
In addition, we explored the effect of IL-13 on both the IL-1 receptor (IL-1R) and TNF-receptor (TNF-R) systems on OA synovial fibroblasts.
|
9616438 |
1998 |
Degenerative polyarthritis
|
0.100 |
Biomarker
|
disease |
BEFREE |
This inhibitory effect of ET-1 was reversed by addition of specific antagonist of ETA receptor. mRNA expression of ETA and ETB receptors was significantly greater in RA fibroblasts stimulated with TNF-alpha or even medium alone than in OA fibroblasts.
|
10090156 |
1999 |
Degenerative polyarthritis
|
0.100 |
Biomarker
|
disease |
BEFREE |
The results indicate that these antiinflammatory cytokines reversed the TNFalpha-induced release of PGE2 by OA synovial fibroblasts, by acting at various levels of the TNFa-dependent signaling cascade.
|
10211885 |
1999 |
Degenerative polyarthritis
|
0.100 |
Biomarker
|
disease |
BEFREE |
OB were isolated from subchondral bone of rheumatoid arthritis (RA), osteoarthritis (OA) and post-traumatic (PT) patients, cultured in vitro in the presence or absence of interleukin 1beta (IL-1beta) and tumor necrosis factor-alpha (TNF-alpha), and assessed for the production, immunolocalization, and mRNA expression of proinflammatory cytokines (IL-1alpha, IL-1beta, TNF-alpha) and alpha and beta chemokines [IL-8, growth related gene product (GRO-alpha), monocyte chemoattractant protein 1 (MCP-1), RANTES, and macrophage inflammatory proteins MIP-1alpha, MIP-1beta].
|
10229398 |
1999 |
Degenerative polyarthritis
|
0.100 |
Biomarker
|
disease |
BEFREE |
Chondrocytes located in cartilage proximal to the macroscopic OA lesions bound more tumor necrosis factor alpha (TNFalpha) and interleukin-1beta (IL-1beta) compared with chondrocytes isolated from morphologically normal cartilage from the same joint.
|
10643716 |
2000 |
Degenerative polyarthritis
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Synovial tissues obtained from 3 patients with HAAP and 3 patients with osteoarthritis (OA) were examined for the expression of tumor necrosis factor-alpha (TNF-alpha) mRNA, HTLV-I tax/rex mRNA, and number of CD68 by in situ reverse transcription assay and immunohistochemistry.
|
10782809 |
2000 |
Degenerative polyarthritis
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Decalcified paraffin-embedded sections from 10 patients with RA and five patients with osteoarthritis (OA) were examined for the immunolocalization of cathepsins B, K and L and the localization of messenger RNAs for interleukin 1beta (IL-1beta), tumour necrosis factor alpha (TNF-alpha) and matrix metalloproteinase 9 (MMP-9).
|
11285370 |
2001 |
Degenerative polyarthritis
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
However, regulation of chemokine expression by IL-1beta and TNFalpha differed between normal and OA chondrocytes.
|
11352237 |
2001 |
Degenerative polyarthritis
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
RA ST cells possessed significantly higher TACE-like enzymatic activity than OA ST. A synthetic TACE inhibitor significantly reduced the release of TNF-alpha and p75 TNF receptor from RA ST cells.
|
11508576 |
2001 |
Degenerative polyarthritis
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Since the increased levels of catabolic enzymes, prostaglandins, nitric oxide (NO) and other markers in OA fluids and tissues appear to be related to elevated levels of IL-1 and TNF-alpha, therapies that interfere with the expression or actions of these cytokines are most promising.
|
11728217 |
2001 |
Degenerative polyarthritis
|
0.100 |
Biomarker
|
disease |
BEFREE |
To determine and compare the expression of cathepsins K and S proteins in joints with rheumatoid arthritis (RA) and osteoarthritis (OA) and to determine the effect of interleukin-1 beta (IL-1 beta) and tumor necrosis factor alpha (TNF alpha) on the expression of cathepsin K in fibroblast-like synoviocytes.
|
11920402 |
2002 |
Degenerative polyarthritis
|
0.100 |
Biomarker
|
disease |
BEFREE |
In this study, the inflammatory effect of TGF-beta1 in rheumatoid arthritis (RA) was investigated using cultured fibroblast-like synoviocytes (FLS) from RA and osteoarthritis (OA) patients, as well as non-arthritic individuals. mRNA expressions of IL-1beta, tumour necrosis factor (TNF)-alpha, IL-8, macrophage inflammatory protein (MIP)-1alpha and metalloproteinase (MMP)-1 were increased in RA and OA FLS by TGF-beta1 treatment, but not in non-arthritic FLS.
|
11966774 |
2002 |
Degenerative polyarthritis
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
We have previously shown that the inflammatory cytokines tumour necrosis factor alpha (TNF-alpha) and interleukin (IL) 6 or IL-1beta are up-regulated in chondrocytes of patients with osteoarthritis (OA).
|
12048293 |
2002 |
Degenerative polyarthritis
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Chondrocytes from the knee cartilage of 25 postmenopausal osteoarthritic (OA) patients were cultured under various conditions: 0 pg/mL, 50 pg/mL, 500 pg/mL, and 5,000 pg/mL of 17beta-estradiol, with or without 10-1,000 pg/mL of either interleukin (IL)-1beta or tumor necrosis factor alpha (TNFalpha).
|
12684836 |
2003 |
Degenerative polyarthritis
|
0.100 |
Biomarker
|
disease |
BEFREE |
TNF-a stimulation induced IL-6 secretion by RA SFB (3-fold) and OA SFB (4-fold), as well as MMP-1 secretion (RA, 85-fold; OA, 29-fold), with significant differences between RA and OA.
|
12913922 |
2003 |
Degenerative polyarthritis
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Furthermore, the pro-inflammatory cytokines IL-1beta and TNF-alpha, known to be present in synovium and cartilage of patients with osteoarthritis, stimulate the production of active BMP-2.
|
12925611 |
2003 |
Degenerative polyarthritis
|
0.100 |
Biomarker
|
disease |
BEFREE |
In vitro, the intracellular expression of galectin 3 in RA and OA synovial fibroblasts after modulation with tumor necrosis factor alpha (TNFalpha), interleukin-1beta (IL-1beta), and anti-CD40 monoclonal antibodies was measured by flow cytometry.
|
14558084 |
2003 |
Degenerative polyarthritis
|
0.100 |
Biomarker
|
disease |
BEFREE |
Importantly, the expression levels of GRB2 and FLICE2 genes were remarkably enhanced in RA synoviocytes but not in OA synoviocytes in response to tumor necrosis factor (TNF)-alpha treatment.
|
14687710 |
2003 |
Degenerative polyarthritis
|
0.100 |
Biomarker
|
disease |
BEFREE |
Interleukin-1 (IL-1), tumor necrosis factor-alpha (TNF-alpha), and matrix metalloproteinases (MMPs) play important roles in the pathogenesis of osteoarthritis (OA).
|
15316669 |
2004 |
Degenerative polyarthritis
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
FLS from RA and OA tissue expressed low baseline levels of cryopyrin transcripts that were induced by tumour necrosis factor alpha (TNFalpha).
|
15498798 |
2005 |
Degenerative polyarthritis
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Levels of mRNA expression of IL-18, IL-8, and TNF-alpha were significantly higher in the synovial membranes of AOSD patients compared with those in osteoarthritis controls.
|
15517632 |
2004 |