Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Caspase 8-null neuroblastoma cells were resistant to death receptor- and doxorubicin-mediated apoptosis, deficits that were corrected by programmed expression of the enzyme.
|
10802708 |
2000 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Caspase-8 silenced N-type invasive NB cell lines LAN-1 and IMR-32 were investigated for their sensitivity to dox, and compared to S-type noninvasive SH-EP NB cells expressing caspase-8.
|
12203125 |
2002 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
CASP8 defects therefore represent an independent event in neuroblastoma, counteracting the N-myc induced susceptibility to apoptosis.
|
12880977 |
2003 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Caspase-8 levels correlate with the expression of signal transducer and activator of transcription 1 in high-grade but not lower grade neuroblastoma.
|
16886176 |
2006 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
A missense mutation was detected at codon 96, GCT (Alanine) to GTT (Valine), of the caspase 8 gene in one of the NB cell lines lacking caspase 8 expression.
|
11466626 |
2001 |
Childhood Neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
A missense SNP in exon 10 of the CASP8 gene SNP D302H was associated with worse overall and event-free survival in patients with MYCN-amplified neuroblastoma tumors.
|
25502557 |
2014 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
An interferon-sensitive response element is involved in constitutive caspase-8 gene expression in neuroblastoma cells.
|
17036321 |
2007 |
Childhood Neuroblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
As frequent methylation of the CASP8 gene has also been reported in neuroblastoma, we investigated whether RASSF1A and CASP8 methylation were independent or related events.
|
11709729 |
2001 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
By revealing no correlation between caspase-8 expression and MYCN amplification or other established variables of aggressive disease, our findings in a large cohort of neuroblastoma patients show that inactivation of caspase-8 is not a characteristic feature of aggressive neuroblastoma.
|
17047064 |
2006 |
Childhood Neuroblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Contrarily, the presence of the -652 6N Del or the CASP8 302His variant was reported to be an unfavorable prognostic factor in colorectal cancer or neuroblastoma.
|
27507139 |
2016 |
Childhood Neuroblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
Detection of CpG island hypermethylation of caspase-8 in neuroblastoma using an oligonucleotide array.
|
19260109 |
2009 |
Childhood Neuroblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
Epigenetic alterations have been described as well: caspase-8 (CASP8) and RAS-association domain family 1 isoform A (RASSF1A) DNA-methylation are important events for the development and progression of neuroblastoma.
|
21725203 |
2011 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Exogenous interferon-γ upregulated expression of caspase-8 in 3 of 4 neuroblastoma cell lines and increased the contribution of TRAIL to NK cytotoxicity against 2 of the 3 lines; however, relatively little inhibition of cytotoxicity was observed when activated NK cells were treated with an anti-interferon-γ neutralizing antibody.
|
23719242 |
2013 |
Childhood Neuroblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
Frequent promoter hypermethylation of RASSF1A and CASP8 in neuroblastoma.
|
17064406 |
2006 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Furthermore, gemcitabine-induced apoptosis was observed irrespective of the caspase-8 status of neuroblastoma cells, which indicates that apoptosis depends on the mitochondrial pathway.
|
15867251 |
2005 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Furthermore, the methylation pattern of 14.3.3sigma, RASSF1A and of an intragenic segment of CASP8 was significantly different between MYCN amplified and single copy neuroblastoma suggesting a specific role of epigenetic alterations in aggressive neuroblastoma.
|
16044164 |
2005 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Here we show that four different retinoic acid (RA) derivatives also increase caspase-8 protein expression in neuroblastoma, medulloblastoma and small cell lung carcinoma cell lines.
|
18342014 |
2008 |
Childhood Neuroblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
However, approximately 20% of the neuroblastoma cell lines with methylated CASP8 alleles are also highly resistant to staurosporine (STS)- and radiation-induced cell death, presumably because cytochrome c is not released from mitochondria.
|
11896617 |
2002 |
Childhood Neuroblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
However, the IFN-gamma restoration of caspase 8 in some NB cells revealed persistent TRAIL resistance in most NB cell lines examined.
|
12615731 |
2003 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Our results show that restoration of active caspase-8 expression in a caspase-8-deficient NB cell line is necessary and sufficient to fully restore TRAIL sensitivity.
|
15094781 |
2004 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Re-expression of caspase-8 in neuroblastoma cells lacking endogenous caspase-8 expression was found to promote cell adhesion to extracellular matrix and to activate adhesion-dependent signaling pathways, such as the Erk kinase cascade.
|
18089800 |
2007 |
Childhood Neuroblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
Sixty-two NBs (45 primary tumors and 17 NBs at relapse) were studied in terms of the methylation status of 19 genes (p15INK4a, p16INK4a, p14ARF, APC, RB1, RASSF1A, BLU, FHIT, RARbeta, INI1, TIMP3, NF2, MGMT, DAPK, FLIP, ECAD, CASP8, and the receptors DcR1 and DcR2).
|
17570703 |
2008 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Subcellular fractionation analysis revealed caspase-8 activity in both cytosol and plasma membrane fractions in both NB7 reconstituted caspase-8 cell lines, as well the LNCaP prostate cancer cells.
|
23456622 |
2013 |
Childhood Neuroblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
Subsequently others have also shown that caspase-8 is silenced by methylation in neuroblastoma and peripheral neural ectodermal tumors, and that the caspase-9 regulator Apaf-1 is silenced by methylation in melanoma cell lines and patient samples.
|
11511973 |
2001 |
Childhood Neuroblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
The authors found that interferon-gamma induces caspase-8 expression in neuroblastoma cells irrespective of the gene silenced by hypermethylation of caspase-8 promoter.
|
15065018 |
2004 |