Genomic DNA was isolated from whole blood for the analysis of <i>VEGF-A</i> (rs2010963, 1570360, rs699947), <i>ICAM-1</i> (rs5498, rs1799969) SNPs and from tumor tissue for the detection of genomic variants in <i>KRAS</i>, <i>NRAS</i>, <i>BRAF</i> genes.
After stratifying by tumor molecular subtype, SNP associations observed for colon cancer were: VEGFA rs2010963 with CIMP+ colon tumors; FLT1 rs4771249 and rs7987649 with TP53; FLT1 rs3751397, rs7337610, rs7987649, and rs9513008 and KDR rs10020464, rs11941492, and rs12498529 with MSI+ and CIMP+/KRAS2-mutated tumors.
Blood and tumor tissue from 83 patients with NSCLC were examined for VEGF -460T/C (rs833061) and VEGF +405G/C (rs2010963) SNPs using the SNaPshot method.