We report that human leukocyte interferon preparations increase the expression of beta 2-microglobulin by 100-200% on the surface of normal fibroblast and melanoma cell lines sensitive to interferon.
Linkage group V of platyfishes and Swordtails of the genus Xiphophorus (Poeciliidae): linkage of loci for malate dehydrogenase-2 and esterase-1 and esterase-4 with a gene controlling the severity of hybrid melanomas.
Binding studies indicated the presence of 2,980 +/- 170 receptors per cell, each with an apparent Kd of (8.4 +/- 1.3) X 10(-11) M. Results from competitive binding studies suggested that Hs294T cells possess at least two types of IFN receptors: one which binds IFN-alpha A and IFN-beta 1 and another to which IFN-gamma binds.
Binding studies indicated the presence of 2,980 +/- 170 receptors per cell, each with an apparent Kd of (8.4 +/- 1.3) X 10(-11) M. Results from competitive binding studies suggested that Hs294T cells possess at least two types of IFN receptors: one which binds IFN-alpha A and IFN-beta 1 and another to which IFN-gamma binds.
Two of the major protein species (gp88 and gp130) were common to all four cell lines used (HeLa-cervical carcinoma, T47D-breast carcinoma, and HMB2 and SK1477-two melanoma cell lines).
Standard carcinoembryonic antigen(s) (CEA) obtained commercially, and CEA preparations from cell membranes and spent media of cultured human malignant melanotic melanoma (HMMC-ShAc and HMMC-ShAm) and of human colon adenocarcinomas (Levo c, Levo m, SW-403 c and SW-403 m) suppressed human peripheral blood lymphocyte (PBL).
Two of the major protein species (gp88 and gp130) were common to all four cell lines used (HeLa-cervical carcinoma, T47D-breast carcinoma, and HMB2 and SK1477-two melanoma cell lines).
Two of the major protein species (gp88 and gp130) were common to all four cell lines used (HeLa-cervical carcinoma, T47D-breast carcinoma, and HMB2 and SK1477-two melanoma cell lines).
In addition, IFN-gamma induced the expression of another class II antigen, HLA-DC, on a HLA-DR+ and -DC-melanoma cell line and to a lower level on a -DR- and -DC-melanoma line.
MoAb PAL-M1 reacted with all 15 melanoma metastases (MM), with 14 of 19 primary cutaneous melanomas (PCM), 9 of 35 dysplastic nevi (DN), and 2 of 26 NN.
MoAb PAL-M1 reacted with all 15 melanoma metastases (MM), with 14 of 19 primary cutaneous melanomas (PCM), 9 of 35 dysplastic nevi (DN), and 2 of 26 NN.
MoAb PAL-M1 reacted with all 15 melanoma metastases (MM), with 14 of 19 primary cutaneous melanomas (PCM), 9 of 35 dysplastic nevi (DN), and 2 of 26 NN.
We propose that p97 be renamed melanotransferrin to denote its original identification in melanoma cells and its evolutionary relationship to serotransferrin and lactotransferrin, the other members of the transferrin superfamily.
Further evidence for differences between the melanoma and fibroblastic cell adhesion systems was obtained by examining adhesion to proteolytic fragments of fibronectin.
We now report on a family of fibronectin mRNAs identified by Northern blotting analysis in two normal human fibroblast strains (HEL 299 and Flow 7000) and five transformed cell lines (8387 and HT-1080, fibrosarcomas; G-361, melanoma; JEG-3, choriocarcinoma; and RD, rhabdomyosarcoma).
We propose that p97 be renamed melanotransferrin to denote its original identification in melanoma cells and its evolutionary relationship to serotransferrin and lactotransferrin, the other members of the transferrin superfamily.
We propose that p97 be renamed melanotransferrin to denote its original identification in melanoma cells and its evolutionary relationship to serotransferrin and lactotransferrin, the other members of the transferrin superfamily.