AQP-1 protein expression was significantly associated with CT (<i>p</i> = .001) in all groups; however, there was a significant negative correlation (<i>p</i> = .029) between AQP-1 protein expression and axial length in the REC groups.<b>Conclusions</b>: AQP-1 protein expression in the choroid was upregulated following recovery of FD myopia in guinea pigs, and these changes correlated with alterations in CT and axial length.