Our findings suggest that PLCγ1 is a critical regulator of colitis and colorectal cancer and could further help in the development of therapy for colitis-associated cancer.
We used robotized capillary isoelectric focusing as well as conventional immunoblotting for the comprehensive analysis of epidermal growth factor receptor signaling pathways converging on extracellular regulated kinase 1/2 (ERK1/2), AKT, phospholipase Cγ1 (PLCγ1) and c-SRC in normal mucosa compared with CRC stage II and IV.
Additionally, immunoblot analyses revealed that the level of PLC-gamma 1 expression was considerably higher in 9 of 11 colorectal carcinomas than in adjacent normal colorectal tissues.
Although factors that might influence the level of PLC-gamma 1 expression in colorectal carcinomas still remain obscure, the fact that most colorectal carcinomas display elevated levels of PLC-gamma 1 expression implies that PLC-gamma 1 may play an important role in proliferation of colorectal carcinoma cells.