Commonly described mutations include mutations in epidermal growth factor (<i>EGFR</i>) & <i>BRAF</i> and translocations in anaplastic lymphoma kinase (<i>ALK</i>) & rat osteosarcoma (<i>ROS1</i>).
The objective of this study was to explore the effect and mechanism of downregulating epidermal growth factor-like domain 7 expression in a human osteosarcoma cell line on the biological function of co-cultured human umbilical vein endothelial cells.
The association of the expression of HER receptors in osteosarcoma with clinicopathological parameters was analyzed using χ<sup>2</sup> test and Fishers exact test.
Western blotting and a quantitative real-time RT-PCR assay developed for T-cadherin showed that estradiol, progesterone, EGF, dexamethasone and factors in serum were involved in transcriptional and post-transcriptional regulation of T-cadherin in human osteosarcoma cells; the effects observed were opposite to those described for T-cadherin's ligand, adiponectin.
Human epidermal growth factor (Her-2/neu) expression in 25 initial pretreatment osteosarcoma biopsies and 12 posttreatment pulmonary metastatic osteosarcoma resection specimens was assessed by standard immunohistochemical techniques on formalin-fixed paraffin-embedded tissue.