Compounds 40 and 41 exhibited high brain permeability in the PAMPA-BBB assay, significant displacement of propidium iodide from the peripheral anionic site (PAS) of AChE, and were devoid of neurotoxicity towards SH-SY5Y neuroblastoma cell lines up to the maximum tested concentration of 80 μM.
We demonstrate for the first time that Cassia tora fraction prevents Aβ <sub>1-42</sub> aggregation, inhibits acetylcholinesterase and alleviates Aβ <sub>1-42</sub> -induced oxidative stress in human neuroblastoma cells.
The current study evaluated the safety profile of EA in the SH-SY5Y human neuroblastoma cell line, performing anti-oxidative effect by DPPH assay, and evaluating anti-AchE (acetylcholinesterase) effect against AchE enzyme from Electrophorus electricus.
Human neuroblastoma SH-SY5Y cells were treated with H<sub>2</sub>O<sub>2</sub> (1-1000µM) for 24h and AChE activity and AChE and cytochrome c levels were evaluated.
These results suggest that AChE plays an important role in the procession of neuroblastoma cell apoptosis and favor the association between AChE and neuronal apoptosis in AD.
In addition, a constitutively active mutant of activating transcription factor-1 (ATF-1) was able to turn on human AChE promoter by approximately 4-fold when they were co-expressed in the neuroblastoma cells.