The optical density value of T98G is negatively correlated with the levels of miR-222 (R<sup>2</sup> = 0.7848, P < 0.05), and positively correlated with the levels of GAS5 (R<sup>2</sup> = 0.6886, P < 0.05).
Additionally, the functions of miR-221/222 on glioblastoma cell angiogenesis were measured in vivo with microvessel density assayed. miR-221 and miR-222 were expressed at a high level and SOCS3 was at a low level in glioblastoma.
In this study, we designed adenovirally-expressed shRNAs that functionally co-repressed the expression of miR-221 and miR-222, which are related to glioblastoma, to overcome the low efficiency of gene therapy.