Then, AMC-HN-8 cells were transfected with miR-141 mimic, miR-141 inhibitor and HOXC6-siRNA to investigate specific role of miR-141, HOXC6 and TGF-β signaling pathway in laryngeal cancer in vivo and in vitro.
The aim of the present study was to analyze early and late activation of T-lymphocytes related to Epstein-Barr virus (EBV) infection by the expression of markers of activation (CD69, CD25) on the surface of T-lymphocytes (CD3<sup>+</sup>, CD4<sup>+</sup>, CD8<sup>+</sup>) in patients bearing laryngeal cancer according to absence/presence immunoglobulin G antibodies to EBV nuclear antigen (EBNA1).
Then, AMC-HN-8 cells were transfected with miR-141 mimic, miR-141 inhibitor and HOXC6-siRNA to investigate specific role of miR-141, HOXC6 and TGF-β signaling pathway in laryngeal cancer in vivo and in vitro.
The aim of the present study was to analyze early and late activation of T-lymphocytes related to Epstein-Barr virus (EBV) infection by the expression of markers of activation (CD69, CD25) on the surface of T-lymphocytes (CD3<sup>+</sup>, CD4<sup>+</sup>, CD8<sup>+</sup>) in patients bearing laryngeal cancer according to absence/presence immunoglobulin G antibodies to EBV nuclear antigen (EBNA1).
To identify the putative relevance of autophagy in laryngeal cancer, we performed an immunohistochemistry study to analyze the expression of the proteins involved in this process, namely, LC3, ATG5 and p62/SQSTM1.
Overall, our results suggest that i) cytoplasmic p62 and PTOV1 can be considered prognostic markers in laryngeal cancer, ii) the acquisition of resistance seems to be related to PTOV1 and autophagy-related protein overexpression, iii) by increasing autophagy, PTOV1 might contribute to resistance in this model and iv) the expression of autophagy-related proteins could classify a subgroup of laryngeal cancer patients who will benefit from a therapy based upon autophagy inhibition.
Assessment of these genes in clinical sub-cohorts of TCGA indicated that early stage tongue (MTFR1, C8ORF33, OTUD6B) and laryngeal cancers (TWISTNB, KLHL13 and UBE2Q1) were defined by distinct prognosticators.
Overall, our results suggest that i) cytoplasmic p62 and PTOV1 can be considered prognostic markers in laryngeal cancer, ii) the acquisition of resistance seems to be related to PTOV1 and autophagy-related protein overexpression, iii) by increasing autophagy, PTOV1 might contribute to resistance in this model and iv) the expression of autophagy-related proteins could classify a subgroup of laryngeal cancer patients who will benefit from a therapy based upon autophagy inhibition.
Assessment of these genes in clinical sub-cohorts of TCGA indicated that early stage tongue (MTFR1, C8ORF33, OTUD6B) and laryngeal cancers (TWISTNB, KLHL13 and UBE2Q1) were defined by distinct prognosticators.
Overall, our results suggest that i) cytoplasmic p62 and PTOV1 can be considered prognostic markers in laryngeal cancer, ii) the acquisition of resistance seems to be related to PTOV1 and autophagy-related protein overexpression, iii) by increasing autophagy, PTOV1 might contribute to resistance in this model and iv) the expression of autophagy-related proteins could classify a subgroup of laryngeal cancer patients who will benefit from a therapy based upon autophagy inhibition.
The risk coefficients in the multivariate Cox analysis revealed that LINC02154 and MNX1-AS1 are risk factors for laryngeal cancer, whereas MYHAS and LINC01281 appear to be protective factors.
Radiation not only has the similar effect on laryngeal cancer as SHIP2 knockdown, but also causes significant cell cycle G2 arrest, all of which can be significantly enhanced by SHIP2 knockdown.
Overall, our results suggest that i) cytoplasmic p62 and PTOV1 can be considered prognostic markers in laryngeal cancer, ii) the acquisition of resistance seems to be related to PTOV1 and autophagy-related protein overexpression, iii) by increasing autophagy, PTOV1 might contribute to resistance in this model and iv) the expression of autophagy-related proteins could classify a subgroup of laryngeal cancer patients who will benefit from a therapy based upon autophagy inhibition.
Serum matrix metalloproteinase 8 and tissue inhibitor of metalloproteinase 1: Potential markers for malignant transformation of recurrent respiratory papillomatosis and for prognosis of laryngeal cancer.