Gingival samples from 18 patients with GAgP and 10 healthy subjects were collected and the level of miR-146a and its targets, including necrosis factor-alpha, interleukin-1beta, and interleukin-6, were assessed using real-time PCR.
CP was characterized by increased expression of genes related to responses to external stimuli and an underexpression of immune system-related genes. qRT-PCR analysis confirmed microarray results, that killer cell immunoglobulin (Ig)-like receptor, two Ig domains and long cytoplasmic tail 4; interleukin-6; and selectin E were more highly expressed in patients from the GAgP group compared with the CP and H groups.
CP was characterized by the increased expression of genes related to responses to external stimuli and an under-expression of immune system-related genes. qRT-PCR analysis confirmed the microarray results, that KIR2DL4, IL6 and SELE were highly expressed in GAgP than CP or H patients.