In an attempt to identify <i>U2af1</i>(S34F)-cooperating changes that promote leukemogenesis, we combined <i>U2af1</i>(S34F) with <i>Runx1</i> deficiency in mice and further treated the mice with a mutagen, <i>N</i>-ethyl-<i>N</i>-nitrosourea (ENU).
The locus of rs974120 shows marks of transcriptional activity in leukemia according to ENCODE data. rs10251201 (7p21.3), rs9318227 (13q22.1), and rs10405859 (19q13.32) were associated with markers related to leukemogenesis and immune and inflammatory responses.
In contrast, p27 mutations that enhance its stability (p27(T187A)) or nuclear retention (p27(S10A)) attenuate leukemogenesis over wild-type p27, validating the tumor-suppressor function of nuclear p27 in CML.