|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Cell cycle and apoptosis assay were analyzed using flow cytometer Accuri C6 and analysis of proteins as retinoblastoma Rb and kinases Cdk4/6 were analyzed by western blotting.
|
31490285 |
2020 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Based on our own results, we propose that maintaining or re-instating pRb function using CDK4/6 inhibitors are potentially effective treatments of diet-induced obesity (DIO).
|
31629614 |
2020 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Dysregulation of retinoblastoma (Rb) signaling pathway have been established as a requirement for glioblastoma (GBM) initiation and progression, which suggests that blockade of CDK4/6-Rb signaling axis for GBM treatment.
|
31787897 |
2019 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
SCCOHT patient tumors are deficient in cyclin D1 yet retain the retinoblastoma-proficient/p16<sup>INK4a</sup>-deficient profile associated with positive responses to CDK4/6 inhibitors.
|
30718512 |
2019 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
We identify CDK2-dependent phosphorylation of RB as an effector of MAPK signalling and critical mediator of resistance to inhibition of CDK4 and CDK6.
|
31043741 |
2019 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Structural and biochemical data revealed that binding of phosphorylated p27 (phosp27) to CDK4 altered the kinase adenosine triphosphate site to promote phosphorylation of the retinoblastoma tumor suppressor protein (Rb) and other substrates.
|
31831640 |
2019 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
CDK4/Rb signaling pathway were determined by western blot and Immunofluorescence assay.
|
30808351 |
2019 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
CDK4/6 inhibitors specifically block the transition from the G1 to the S phase of the cell cycle by dephosphorylation of the retinoblastoma tumor suppressor protein.
|
30959874 |
2019 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Although CDK4/6 inhibitors mainly target the cyclin D-CDK4/6-retinoblastoma tumor suppressor protein (RB) axis, little is known about the clinical impact of inhibiting phosphorylation of other CDK4/6 target proteins.
|
30343527 |
2019 |
|
Retinoblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
In addition, we searched for other retinoblastoma pathway gene alterations (CDK4 amplification and RB1 homozygous deletion) in a subset of patients.
|
31832685 |
2019 |
|
Retinoblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
WGA and SBA reduced levels of B cell lymphoma-2 (Bcl-2), phosphorylation of Bcl-2-associated death promoter (Bad), cyclin-dependent kinase 4 (Cdk4), and phosphorylation of the retinoblastoma (Rb).
|
31231312 |
2019 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Here, we use drug screens to identify agents specifically antagonized by the retinoblastoma tumor suppressor (RB) using CDK4/6 inhibitors.
|
29386107 |
2018 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
In Astrocytoma, IDH-mutant, PIK3R1 mutations (P = 0.0014) and altered retinoblastoma pathway genes (RB1, CDKN2A, and CDK4) (P = 0.013) were independent predictors of poor survival.
|
29016839 |
2018 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
To explore the possible anti-lung cancer effects of PL, A549 cells were treated with PL (0-40 μM) for 24 h. Alterations in the expression of cell cycle-associated proteins (cyclin D1, cyclin-dependent kinase 4 (CDK4), CDK6 and retinoblastoma (Rb)) and intracellular signaling molecules (extracellular signal receptor-activated kinase 1/2 (ERK1/2), Akt, p38 and nuclear factor-κB (NF-κB)) were examined in cells following treatment of PL using Western blot analysis.
|
29109039 |
2018 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Compound 50 (FN-1501), which possesses potent inhibitory activities against FLT3, CDK2, CDK4, and CDK6 with IC<sub>50</sub> values in the nanomolar range, shows antiproliferative activities against MV4-11 cells (IC<sub>50</sub>: 0.008 μM), which correlates with the suppression of retinoblastoma phosphorylation, FLT3, ERK, AKT, and STAT5 and the onset of apoptosis.
|
29357250 |
2018 |
|
Retinoblastoma
|
0.100 |
PosttranslationalModification
|
disease |
BEFREE |
Mechanistic investigations showed that LUCAT1 induced cell cycle G1 arrest by regulating the expression of cyclin D1, cyclin-dependent kinase 4, and phosphorylated retinoblastoma transcriptional corepressor 1.
|
30032137 |
2018 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
CDK4 inhibition by either palbociclib or specific small interference RNA (siRNA) exhibited dose-dependent inhibition of osteosarcoma cell proliferation and growth, accompanied by suppression of the CDK4/6-cyclinD-Rb signaling pathway.
|
29452249 |
2018 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Furthermore, we show that CDK4/6 inhibitor-resistant cell lines reactivate the CDK-RB-E2F pathway, but remain sensitive to mTORC1/2 inhibition, suggesting that mTORC1/2 inhibitors may represent an option for patients that have relapsed on CDK4/6 therapy.<i></i>.
|
29483206 |
2018 |
|
Retinoblastoma
|
0.100 |
GeneticVariation
|
disease |
BEFREE |
Alterations in the CDK4/6-RB signaling pathway are common causes of cell cycle dysregulation in many cancers, including glioblastoma.
|
30151703 |
2018 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Loss of retinoblastoma tumor suppressor gene (RB1) function has been suggested as a cardinal feature of breast cancers that are more sensitive to chemotherapy and conversely resistant to CDK4/6 inhibitors.
|
29564743 |
2018 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Our data further indicated that Cdc37 increased the stability of cyclin-dependent kinase 4 (CDK4) to activate the retinoblastoma 1 (RB1) signaling pathway, followed by increased expression of Bcl-2 and Bcl-xL, which ultimately promoted cell survival in CRC.
|
29288563 |
2018 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
These findings enhanced our understanding of the emerging FOXM1 genetic network in myeloma and provided preclinical support for the therapeutic targeting of the FOXM1-NEK2 and CDK4/6-Rb-E2F pathways using small-drug CDK and NEK2 inhibitors.
|
30463534 |
2018 |
|
Retinoblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
These single cells expressed various levels of ER and RB which are involved in cell cycle regulation; and the response to palbociclib treatment relies not only on the ER-cyclin D1-CDK4/6-RB pathway but it is also dependent on elevated levels of ER and/or RB.
|
29963233 |
2018 |
|
Retinoblastoma
|
0.100 |
Biomarker
|
disease |
BEFREE |
Ribociclib is a specific cyclin dependent kinase (Cdk) 4/6 inhibitor that induces G<sub>1</sub> arrest by blocking the formation of cyclin D1-Cdk4/6 complex and inhibiting retinoblastoma (RB) phosphorylation.
|
29789630 |
2018 |
|
Retinoblastoma
|
0.100 |
AlteredExpression
|
disease |
BEFREE |
Cyclin D1 and p27<sup>Kip1</sup> regulate the activity of cyclin D1-CDK4 complexes and retinoblastoma (Rb), and then they stimulate the progression of G1/S transition and tumour cell proliferation.
|
30519351 |
2018 |